Journal: Molecules

Article Title: Pectenovarin, A New Ovarian Carotenoprotein from Japanese Scallop Mizuhopecten yessoensis

doi: 10.3390/molecules25133042

Figure Lengend Snippet: ( A ) The chromatogram at 280 nm and ( B ) HPLC absorbance spectrum from pectenovarin purification using a photodiode array detector (PDA). ( C ) SDS-PAGE of the HPLC fractions. M: size marker; C: the crude extract of the scallop ovary; 1–9: the fraction numbers corresponding to A. ( D ) Absorbance spectrum of pectenovarin at T R = 8.459 min and ( E ) pectenolone in acetonitrile/methanol/water at 50:50:1. The peak maxima of the carotenoid chromophore of pectenovarin are at 479 and 503 nm, while that of pectenolone is at 463 nm. ( F ) The ladder-like bands originally appeared and gradually converged at 95 kDa (band 4) after several freeze-thaw processes.

Article Snippet: We directly applied the KBr extract to gel filtration high-pressure liquid chromatography (HPLC) (BioSec-5), using the above buffer without KBr as eluent, and separated into nine fractions ( A,B); of these, fraction 4 had noticeable pink color, while fraction 5 had very weak pink color.

Techniques: Purification, SDS Page, Marker

Journal: Applied and Environmental Microbiology

Article Title: tRNA-Dependent Aminoacylation of an Amino Sugar Intermediate in the Biosynthesis of a Streptothricin-Related Antibiotic

doi: 10.1128/AEM.00725-16

Figure Lengend Snippet: Enzymatic characterization of rOrf11. Purified rOrf11 was subjected to SDS-PAGE. Proteins were stained with CBB R-250. The relative molecular mass of rOrf11 was estimated by gel filtration chromatography (SunSec Diol; 4 mm; 300 by 4.6 mm; ChromaNik Technologies, Osaka, Japan). Glutamate dehydrogenase (290 kDa), lactate dehydrogenase (142 kDa), enolase (67 kDa), myokinase (32 kDa), and cytochrome c (12.4 kDa) were used as the standard molecular masses.

Article Snippet: The relative molecular mass of rOrf11 was estimated by gel filtration chromatography (SunSec Diol; 4 mm; 300 by 4.6 mm; ChromaNik Technologies, Osaka, Japan).

Techniques: Purification, SDS Page, Staining, Filtration, Chromatography

Journal: The Journal of Biological Chemistry

Article Title: Decreased Expression of ARV1 Results in Cholesterol Retention in the Endoplasmic Reticulum and Abnormal Bile Acid Metabolism *

doi: 10.1074/jbc.M110.165761

Figure Lengend Snippet: Knockdown of hepatic ARV1 affects plasma lipid homeostasis. A and B, FPLC analysis of the plasma lipoproteins of pooled plasma samples derived from four to six mice injected with ASOs biweekly for 1 week (A) or 2 weeks (B). C, measurement of TG secretion rates in mice treated with control ASO or ARV1 ASO. Wild type mice treated with control ASO or ARV1 ASO for a week and mice were injected intraperitoneally with 400 μl of P-407 (1 mg/g) solution at zero time point. Rate of TG production was measured over varying time periods in P-407-treated mice. Two to three separate identical experiments were performed with four to six mice/group per treatment in all experimental groups. Values are expressed as means ± S.E. (error bars) of the mice in the representative experiment. Asterisks denote statistical significant differences from control for ASO-treated animals with p < 0.05 (one asterisk) and p < 0.001 (two asterisks) by unpaired t test. D, hepatic LDLR mRNA levels of mice injected with either control ASO or ARV1 ASO over the time course of 2 weeks.

Article Snippet: Pooled plasma samples (120 μl) from four to six mice were subjected to fast protein liquid chromatography (FPLC) gel filtration (Pharmacia LKB Biotechnology) on two Superose 6 columns at a flow rate of 0.5 ml/min (one run/sample), and fractions of 500 μl each were collected ( 12 ).

Techniques: Knockdown, Clinical Proteomics, Derivative Assay, Injection, Control